DETEKSI GEN pmfA Proteus mirabilis PADA URIN PASIEN INFEKSI SALURAN KEMIH MENGGUNAKAN POLYMERASE CHAIN REACTION (PCR)

DETEKSI GEN pmfA Proteus mirabilis PADA URIN PASIEN INFEKSI SALURAN KEMIH MENGGUNAKAN POLYMERASE CHAIN REACTION (PCR)

Nevi Amelia Yasmin

NPM 113216017

Program Diploma Jurusan Analis Kesehatan STIKES JENDERAL ACHMAD YANI CIMAHI

Email: neviameliayasmin@yahoo.co.id

Abstract

Urinary tract infection (UTI) is one of the most frequent nosocomial infection occurred. According to the National nosocomial infection surveillance (NNIS), the number of cases of health about 35% of all infections. Proteus mirabilisis one of the most important causes of urinary tract infections. This P.mirabilis produce fimbriae and hemagglutinin, Proteus mirabilis fimbriae which includes (PMF). PMF role in the pathogenesis of UTI. PMF gene cluster, which is encoded by 5655 base pair(bp) estimated that there are five polypeptides pmfA, pmfC, pmfD, pmfE, and pmfF. But pmfA geneis a gene that encodes the major fimbria of the PMF.

The method use dis descriptive method. This study aims to detect the presence of P.mirabilis genes pmfA of the patient’s urine UTI in the treatment room Kemuning Hasan Sadikin Hospital. The sample used is urine taken from 5 patients UTI. First inspection conducted by DNA isolation, DNA amplification receipts then PCR (Polymerase Chain Reaction) and electrophoresis.

The results of the study on the detection of gene pmfA P.mirabilis UTI urine electrophoresis using PCR of 5 samples UTI visualization is not obtained from gene pmfA P.mirabilis tape so it can be concluded that based on the results of this study concluded that the PCR was not found of genes pmfA P.mirabilis from urine of UTI patients in Hasan Sadikin Hospital.

 Keywords         : Urinary Tract Infection, PCR, pmfA

113216017_Nevi Amelia Yasmin

Pengaruh Pemberian Kompres Povidon Iodine 10% terhadap Lama Penyembuhan Luka Perineum di BPM Ani Mahmudah Jalan Andanwangi No.15 Lamongan

PENGARUH PEMBERIAN KOMPRES POVIDON IODINE 10% TERHADAP LAMA PENYEMBUHAN LUKA PERINEUM DI BPM ANI MAHMUDAH JALAN ANDANWANGI NO. 15 LAMONGAN

 Putri Wulandari Suwadji

Sekolah Tinggi Ilmu Kesehatan Muhammadiyah Lamongan 2014

ABSTRAK

 Rupture of the perineum occurs almost all first labor and not infrequently also in the next delivery. Perineal wound healing is affected by personal hygiene, nutrition, rest and wound care using antiseptic (povidone iodine 10%). Based on the results of the initial survey, there are still postpartum who experienced delay in healing the wound perineum. The purpose of this research is to know the relation of 10% povidon iodine compress on healing of perineal wound on postpartum mother at BPM Ani Mahmudah Lamongan.

The research design used was pre experiment, the population that is all postpartum giving birth at BPM Ani Mahmudah Lamongan, from May to July. Samples taken were all postpartum who meet inclusion criteria with consecutive sampling technique. Dependent variable is healing of perineal wound and independent variable that is giving 10% povidon iodine compress. Data were taken by interview and observation. Data processing using editing, coding, scoring and tabulating then analyzed using contingency coefficient test with α <0,05.

The results showed half a 50% postpartum was given 10% povidon iodine compress almost completely experienced rapid wound healing. The result of contingency coefficient test (C) = 0,464 p = 0,019 (p <α) so that Ho is rejected, it means there is a relation of 10% povidon iodine compressing to perineum wound healing on postpartum mother.

Reference from this research is need to increase the role of health officer in giving proper information about keeping personal hygiene after delivery and not forgetting 10% povidon iodine compress in order to optimize healing from perineal wound.

 Keywords: giving 10% povidon iodine compress, perineal wound healing.

113216016_putri wulandari s

Gambaran Karakteristik Akseptor KB Suntik Hormonal Di RSIA AULIA. Jakarta Selatan Period Januari-Februari 2014

113216001_siti Hanifah

UJI AKTIVITAS ISOLAT BAKTERIOSIN Lactobacillus sp DALAM MENGHAMBAT PERTUMBUHAN Pseudomonas aeruginosa

UJI AKTIVITAS BAKTERIOSIN ISOLAT Lactobacillus sp DALAM MENGHAMBAT PERTUMBUHAN Pseudomonas aeruginosa

Bias Relati Ronanur

NIM P17334111019

Program D III Jurusan Analis Kesehatan Poltekkes Kemenkes Bandung

Email : biasrelatironanur@gmail.com

 ABSTRACT

 Pseudomonas aeruginosa is an opportunistic pathogen and a bacterial major caused of nosocomial infection. Lactic acid bacteria (LAB) produced metabolite that well known as bacteriocin. Bacteriocin has bactericidal activity, bacteriostatic, and therapeutic effect. The purpose of this research to determine the activity of supernatant bacteriocin Lactobacillus sp in inhibit the growth of P. aeruginosa and to know bacteriocin of Lactobacillus sp which has the highest activities in inhibit the growth of P. aeruginosa. The kind of this research is an experiment using two methods that was agar diffusion and microdilution. The result showed that the supernatant of bacteriocin L. acidophilus, L. bulgaricus, and L. casei with diameter inhibition zone 11.4 ± 0.64 mm, 11.4 ± 0.62 and 9.5 ± 0.28. MIC values ​​of the three samples is 6.25%, MBC value of  L. acidophilus, L. bulgaricus is 12.5%, and L. casei is 25%. The result showed that supernatant bacteriocin of Lactobacillus sp has activity in inhibiting the growth of P. aeruginosa with the highest activity was shown by bacteriocin L. acidophilus and L. bulgaricus. Suggestion for further research to use a single or a combination of bacteriocin then tested the activities to other pathogenic bacteria.

 Keywords        : Bacteriocin, Lactobacillus sp, Pseudomonas aeruginosa

113216006_BiasRelatiRonanur

Hubungan Antara Konsumsi Energi dan Protein dengan Status Gizi lansia di Balai Perlindungan Sosial Tresna Werdha (BPSTW) Ciparay

HUBUNGAN ANTARA KONSUMSI ENERGI DAN PROTEIN DENGAN STATUS GIZI LANJUT USIA DI BALAI PERLINDUNGAN SOSIAL TRESNA WERDHA CIPARAY

Ardy Ahmad Budiman

Jurusan Gizi

Politeknik Kesehatan Kementerian Kesehatan Bandung

Jl. Babakan Loa No.10A, Pasirkaliki, Cimahi Utara, Kota Cimahi, Jawa Barat 40514

email :ardyahmadbudiman@gmail.com 

ABSTRAK

 Lanjut usia merupakan fase kehidupan yang akan dilalui oleh setiap individu. Kondisi kesehatan pada tahap ini sangat ditentukan oleh kualitas dan kuantitas konsumsi zat gizi yang pada akhirnya akan menentukan status gizi seseorang. Status gizi adalah hasil dari keseimbangan antara kebutuhan zat gizi yang terkandung dalam makanan. Tujuan dari penelitian ini adalah untuk mengetahui hubungan antara konsumsi energi dan protein dengan status gizi lansia Di Balai Perlindungan Sosial Tresna Werdha Ciparay.

Desain penelitian yang digunakan dalam penelitian ini adalah Cross Sectional. Populasi pada penelitian ini adalah seluruh lansia yang ada di Balai Perlindungan Sosial Tresna Werdha Ciparay dengan jumlah sampel sebanyak 60 orang. Data yang diambil adalah data umum  sampel yang meliputi umur dan jenis kelamin sampel diperoleh dengan cara wawancara menggunakan kuesioner, kemudian data konsumsi energi dan protein diperoleh dengan dengan cara penimbangan makanan menggunakan formulir food weighing dan timbangan digital.

Hasil penelitian menunjukkan lansia yang konsumsi energinya baik sebanyak 43 orang dan lansia yang konsumsi energinya kurang sebanyak 17 orang. Kemudian lansia yang konsumsi proteinnya baik sebanyak 38 orang dan yang konsumsi proteinnya kurang sebanyak 22 orang. Selain itu, sampel yang status gizinya normal sebanyak 40 orang dan yang status gizinya kurus sebanyak 20 orang. Hasil uji statistik menunjukkan adanya hubungan yang bermakna antara konsumsi energi dengan status gizi (p=0,00), kemudian adanya hubungan yang bermakna antara konsumsi protein dengan status gizi (p=0,00).

Saran: Perlu adanya standar porsi sehingga porsi yang diberikan sesuai dengan kebutuhan lansia, terutama untuk makanan pokok.

Kata kunci : Konsumsi Energi, konsumsi Protein dan Status Gizi Lanjut Usia

113216035_Ardy Ahmad Budiman

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