Day: June 18, 2017

Posted in Deskriptif

DETEKSI GEN pmfA Proteus mirabilis PADA URIN PASIEN INFEKSI SALURAN KEMIH MENGGUNAKAN POLYMERASE CHAIN REACTION (PCR)

Posted on by publichealthisyours

DETEKSI GEN pmfA Proteus mirabilis PADA URIN PASIEN INFEKSI SALURAN KEMIH MENGGUNAKAN POLYMERASE CHAIN REACTION (PCR)

Nevi Amelia Yasmin

NPM 113216017

Program Diploma Jurusan Analis Kesehatan STIKES JENDERAL ACHMAD YANI CIMAHI

Email: neviameliayasmin@yahoo.co.id

Abstract

Urinary tract infection (UTI) is one of the most frequent nosocomial infection occurred. According to the National nosocomial infection surveillance (NNIS), the number of cases of health about 35% of all infections. Proteus mirabilisis one of the most important causes of urinary tract infections. This P.mirabilis produce fimbriae and hemagglutinin, Proteus mirabilis fimbriae which includes (PMF). PMF role in the pathogenesis of UTI. PMF gene cluster, which is encoded by 5655 base pair(bp) estimated that there are five polypeptides pmfA, pmfC, pmfD, pmfE, and pmfF. But pmfA geneis a gene that encodes the major fimbria of the PMF.

The method use dis descriptive method. This study aims to detect the presence of P.mirabilis genes pmfA of the patient’s urine UTI in the treatment room Kemuning Hasan Sadikin Hospital. The sample used is urine taken from 5 patients UTI. First inspection conducted by DNA isolation, DNA amplification receipts then PCR (Polymerase Chain Reaction) and electrophoresis.

The results of the study on the detection of gene pmfA P.mirabilis UTI urine electrophoresis using PCR of 5 samples UTI visualization is not obtained from gene pmfA P.mirabilis tape so it can be concluded that based on the results of this study concluded that the PCR was not found of genes pmfA P.mirabilis from urine of UTI patients in Hasan Sadikin Hospital.

 Keywords         : Urinary Tract Infection, PCR, pmfA

113216017_Nevi Amelia Yasmin

Posted in Kebidanan

Pengaruh Pemberian Kompres Povidon Iodine 10% terhadap Lama Penyembuhan Luka Perineum di BPM Ani Mahmudah Jalan Andanwangi No.15 Lamongan

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PENGARUH PEMBERIAN KOMPRES POVIDON IODINE 10% TERHADAP LAMA PENYEMBUHAN LUKA PERINEUM DI BPM ANI MAHMUDAH JALAN ANDANWANGI NO. 15 LAMONGAN

 Putri Wulandari Suwadji

Sekolah Tinggi Ilmu Kesehatan Muhammadiyah Lamongan 2014

ABSTRAK

 Rupture of the perineum occurs almost all first labor and not infrequently also in the next delivery. Perineal wound healing is affected by personal hygiene, nutrition, rest and wound care using antiseptic (povidone iodine 10%). Based on the results of the initial survey, there are still postpartum who experienced delay in healing the wound perineum. The purpose of this research is to know the relation of 10% povidon iodine compress on healing of perineal wound on postpartum mother at BPM Ani Mahmudah Lamongan.

The research design used was pre experiment, the population that is all postpartum giving birth at BPM Ani Mahmudah Lamongan, from May to July. Samples taken were all postpartum who meet inclusion criteria with consecutive sampling technique. Dependent variable is healing of perineal wound and independent variable that is giving 10% povidon iodine compress. Data were taken by interview and observation. Data processing using editing, coding, scoring and tabulating then analyzed using contingency coefficient test with α <0,05.

The results showed half a 50% postpartum was given 10% povidon iodine compress almost completely experienced rapid wound healing. The result of contingency coefficient test (C) = 0,464 p = 0,019 (p <α) so that Ho is rejected, it means there is a relation of 10% povidon iodine compressing to perineum wound healing on postpartum mother.

Reference from this research is need to increase the role of health officer in giving proper information about keeping personal hygiene after delivery and not forgetting 10% povidon iodine compress in order to optimize healing from perineal wound.

 Keywords: giving 10% povidon iodine compress, perineal wound healing.

113216016_putri wulandari s